Journal: Cell Proliferation

Article Title: Comparative Analysis of the Therapeutic Potential of Extracellular Vesicles Secreted by Aged and Young Bone Marrow‐Derived Mesenchymal Stem Cells in Osteoarthritis Pathogenesis

doi: 10.1111/cpr.13776

Figure Lengend Snippet: Old and young BMSC‐derived EVs showed similar characteristics. (A) Schematic diagram of the EVs isolation procedure from young and old BMSCs (B) Representative nanoparticle tracking analysis of young and old EVs size distribution (C) Cryo‐TEM (upper) images demonstrate the double‐membrane structure of YEV and OEV. Scale bar, 10 nm and transmission electron microscopy (lower) image shows the cup‐shaped structure of EVs. Scale bar, 100 nm. (D) The three‐dimensional topography images of EVs obtained using atomic force microscopy characterisation (E) Protein concentration of YEVs and OEVs (F) Western blot analysis for EV protein markers (Alix and TSG 101) in young BMSC lysate, YEVs, old BMSC lysate and OEVs. (G) Uptake of YEVs and OEVs by primary human chondrocytes were observed under a fluorescent microscope after 24 h of coculture. EVs were stained with PKH26 (red), and DAPI (blue) was used to stain chondrocyte nuclei; scale bar, 20 μm. Data are presented as mean ± SD from three biological replicates for imaging and technical replicates for quantitative analyses (* p ≤ 0.05; ns: not significant).

Article Snippet: Cells were then imaged using an Eclipse TE2000‐U fluorescent microscopy (Nikon, Japan).

Techniques: Derivative Assay, Isolation, Membrane, Transmission Assay, Electron Microscopy, Microscopy, Protein Concentration, Western Blot, Staining, Imaging

Journal: Cell Communication and Signaling : CCS

Article Title: Dissociation of the nuclear WWOX/TRAF2 switch renders UV/cold shock-mediated nuclear bubbling cell death at low temperatures

doi: 10.1186/s12964-024-01866-6

Figure Lengend Snippet: Calcium influx and NO production during UV/cold shock-mediated nuclear bubbling. A , B WWOXf HCT116 cells were exposed to UV and then cold shock at 4 °C for 10 min. Increased NO production was shown (stained with green fluorescent DAF). Red arrows indicate the time of initiation of nuclear bubble formation. C Under similar conditions, WWOXd 4T1 cells had little or no increased production of NO. D Calcium influx was observed in UV-treated HCT116 cells (stained with green fluorescent Fluo-8). Calcium chelator EGTA retarded the bubble formation. E HCT116 cells were added 1 μL of DAF for probing Ca 2+ ion (green) and DAPI (blue) and PI (red) for staining nuclei, followed by exposure to UV (960 mJ/cm 2 ) and cold shock at 4 °C for 10 min, and then time-lapse microscopy at 22 °C

Article Snippet: Also, time-lapse bright field and fluorescent microscopy (Nikon Eclipse TE2000-U, Tokyo, Japan) were carried out to image BCD or POD [ , ].

Techniques: Staining, Time-lapse Microscopy